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The root is pride, aversion from God – the sin was to have knowledge but against the command of God.
We do not know when God constituted Adam and Eve with the preternatural and supernatural gifts, however we know that they were sufficient to remain without mortal sin as long as they cooperated. As the Catechism states:
They had greed to become God. Why otherwise they should not cooperate?1861 Mortal sin is a radical possibility of human freedom, as is love itself. It results in the loss of charity and the privation of sanctifying grace, that is, of the state of grace. If it is not redeemed by repentance and God’s forgiveness, it causes exclusion from Christ’s kingdom and the eternal death of hell, for our freedom has the power to make choices for ever, with no turning back. However, although we can judge that an act is in itself a grave offense, we must entrust judgment of persons to the justice and mercy of God.